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Numerical image processing methods for rotating condenser microscope images processing
 Týč, Matěj ; Martišek, Dalibor (oponent) ; Druckmüller, Miloslav (vedoucí práce)
This thesis describes possible methods of processing images acquired using a transmitted light (aka. widefield) microscope with adjustable condenser aperture. This method is useful when examining thick specimen that have significant third dimension. Classical microscopy is not a very good method to research this type of specimen since the output of widefield microscope incorporates very much information from areas that are not of any interest. This is a serious complication in research of thick specimen (frequently seen in medicine and biology), so when a remedy appeared (confocal microscope), everybody started using it. However, these microscopes are very expensive and have even other drawbacks. The aim is to process images acquired by the method involving rotating condenser aperture using new computer-based techniques. These techniques were not available when scientists begun their research on thick specimen since computers were not so widespread and didn't have enough memory. This method of image processing can be considered as a software substitution of hardware that makes difference between classical and confocal microscopes. In some cases, it can produce exactly the same results.
Úplný záznam
Numerical image processing methods for rotating condenser microscope images processing
 Týč, Matěj ; Martišek, Dalibor (oponent) ; Druckmüller, Miloslav (vedoucí práce)
This thesis describes possible methods of processing images acquired using a transmitted light (aka. widefield) microscope with adjustable condenser aperture. This method is useful when examining thick specimen that have significant third dimension. Classical microscopy is not a very good method to research this type of specimen since the output of widefield microscope incorporates very much information from areas that are not of any interest. This is a serious complication in research of thick specimen (frequently seen in medicine and biology), so when a remedy appeared (confocal microscope), everybody started using it. However, these microscopes are very expensive and have even other drawbacks. The aim is to process images acquired by the method involving rotating condenser aperture using new computer-based techniques. These techniques were not available when scientists begun their research on thick specimen since computers were not so widespread and didn't have enough memory. This method of image processing can be considered as a software substitution of hardware that makes difference between classical and confocal microscopes. In some cases, it can produce exactly the same results.
Úplný záznam

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